Semi-automated microseeding of nanolitre crystallization experiments.
نویسندگان
چکیده
A simple semi-automated microseeding procedure for nanolitre crystallization experiments is described. Firstly, a microseed stock solution is made from microcrystals using a Teflon bead. A dilution series of this microseed stock is then prepared and dispensed as 100 nl droplets into 96-well crystallization plates, facilitating the incorporation of seeding into high-throughput crystallization pipelines. This basic microseeding procedure has been modified to include additive-screening and cross-seeding methods. Five examples in which these techniques have been used successfully are described.
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عنوان ژورنال:
- Acta crystallographica. Section F, Structural biology and crystallization communications
دوره 64 Pt 1 شماره
صفحات -
تاریخ انتشار 2008